ВПЛИВ СТИМУЛЯТОРІВ РОСТУ МІКРОБНОГО ПОХОДЖЕННЯ НА РОЗВИТОК ВЕРМИКУЛЬТУРИ

N. MITINA; I. ZUBAREVA; T. SKLIAR; N. MALINOVSKA; V. SYTAR; A. KUDRIAVTHCEV

doi:10.15407/scine20.04.061

Authors

N. MITINA Ukrainian State University of Chemical Technology https://orcid.org/0000-0002-5384-7040
I. ZUBAREVA Oles Honchar Dnipro National University https://orcid.org/0000-0002-8626-6699
T. SKLIAR Oles Honchar Dnipro National University https://orcid.org/0000-0003-0224-2460
N. MALINOVSKA Ukrainian State University of Chemical Technology https://orcid.org/0000-0001-6745-075X
V. SYTAR Ukrainian State University of Chemical Technology https://orcid.org/0000-0003-0351-945X
A. KUDRIAVTHCEV Ukrainian State University of Chemical Technology https://orcid.org/0000-0002-6299-2530

DOI:

https://doi.org/10.15407/scine20.04.061

Keywords:

Keywords: vermiculture, Eisenia foetida culture, growth stimulants, culture fluid, substrate, biomass.

Abstract

Introduction. The need to implement advanced technologies for obtaining a suffi cient amount of high-quality protein in Ukraine is an aspect of food security. Biotechnology of vermiculture is one of the ways to transform agricultural raw materials of plant and animal origin into good-quality food products.
Problems Statement. The search for ways to increase the productivity of protein biosynthesis in the process of vermiculture is an urgent problem.
Purpose. The purpose is to study the effect of microbial-origin stimulators in the nutritional substrate on the reproductive properties and biomass accumulation of Eisenia foetida.
Materials and Methods. Conventional and advanced physicochemical, microbiological, and technological research methods. Stimulators of microbial origin have been obtained by deep cultivation of Streptomyces recifensis var. lyticus 2435. The effect of stimulators has been studied by determining the biometric indicators of Eisenia foetida culture; the composition of the microbiota of the substrates has been investigated by the method of microscopy.
Results. The proposed growth stimulators are a complex of lytic enzymes (muramidase, 5 types of endopeptidase, 2 types of glycosidase) and accompanying enzymes (proteases, amylases). The maximum indicators have been found in the presence of 0.5% raw biomass of Streptomyces recifensis var. lyticus 2435 in a proportion of 1 : 15 to the dry matter of the nutrient substrate, the microbial composition of which changes against the initial values: the total number decreases by 13.38%, for the bacteria, by 13.92%, for the micromycetes, and by 31.04%, for the anaerobes. The number of representatives of the genus Streptomyces increases 2.17 times; there are no bacteria of the genii Enterobacter and Staphylococcus in the experimental samples. This fact is probably explained by the enzymatic activity of the digestive system of Е. foetida.
Conclusions. The studied stimulators of microbial origin have a positive effect on the nutrient substrate fermentation processes and on the reproductive and growth functions of vermiculture.

Downloads

Download data is not yet available.

References

Rodriguez-Campos, J., Dendooven, L., Alvarez-Bernal, D., Contreras-Ramos, S. (2014). Potential of earthworms to accelerate removal of organic contaminants from soil: A review. Soil Ecology apple, 79, 10—25. https://doi.org/10.1016/j.apsoil.2014.02.010.

Kharchyshyn, V. M., Melnichenko, O. M., Vered, P. I., Zlochevsky, M. V. (2013). Innovations in the growing problems of utilization of organic wastes using the vermiculture method Collection of scientifi c works. Technology of production and processing of animal products: collection of scientifi c works, 10(105), 64—68. URL: http://nbuv.gov.ua/UJRN/ tvppt_2013_10_21 (Last accessed: 20.02.2024) [in Ukrainian].

Stom, D. I., Babin, V. A., Vyatchina, V. A. (2009). On the possibility of enhancing the immune properties of earthworms. Bulletin of the Ulyanovsk State Agricultural Academy, 5, 74—78 [in Russian].

Liu, Z., Wang, J., Zhang, J., Yu, В., Niu, В. (2012). An extract from the earthworm Eisenia fetida non-specifi cally inhibits the activity of infl uenza and adenoviruses. Journal of Traditional Chinese Medicine, 32(4), 657—663. https://doi.org/10.1016/S0254-6272(13)60088-6 .

Karomatov, I. D. (2013). Dog worms — stagnation in ancient and current folk medicine and prospects for stagnation in scientifi c medicine. Current problems of humanities and natural sciences, 7, 2—4 [in Russian].

Matausiyts-Pysl, M., Cupiс, Н., Kasuba, V., Mikecin, А., Grdisa, М. (2010). Tissue extract from Eisenia foetida as a woundhealing agent. Eur. Rev. Med. Pharmacol. Sci., 14(3), 177—184. URL: https://pubmed.ncbi.nlm.nih.gov/20391955/ (Last accessed: 20.02.2024)

Perevozchykov, E. N., Safonova, G. M. (2006). Infusion of “Vermin” ointment on the phagocytic activity of neutrophils during experimental wound infection. Medical immunology, 2 (8), 166—170 [in Russian].

Myra, G., Tereziya, H. (2007). Glycolipoprotein extract of Eisenia fetida (G-90): source of biologically active molecules. European Journal of Soil Biology, 43, 104—109. https://doi.org/10.1016/j.ejsobi.2007.08.055.

Pundir, C. S. (2015). Enzyme nanoparticles.

Titov, I. N., Usoev, V. M. (2012). Vermiculture as a renewable source of animal protein from organic waste. Bulletin of Tomsk State University. Biology, 2(18), 74—80 [in Russian].

Romanova, E. M., Mukhitova, M. E., Titova, E. B. (2014). Fertilized and uncooked rice in the microbiocenosis of the com mercial vermiculture Eisenia fetida Andrei and the natural analogue of Eisenia fetida. International scientifi c and research journal, 3, 64—70 [in Russian].

Edwards, K. A., Arancon, N. K., Sherman, R. L. (2010). Vermiculture technology: earthworms, organic waste and environmental management. CRS Press, Taylor and Francis Group. https://doi.org/10.1201/b10453.

Sitar, V. I., Anisimov, V. M., Mitina, N. B., Garmash, S. M. (2018). Research into biodegradation of linear copolyurethane blocks fi lled by stroke in the process of vermicultivation. Issues of Chemistry and Chemical Technology, 6, 133—138. https://doi.org/10.32434/0321-4095-2018-121-6-133-138 [in Ukrainian].

Chachina, S., Salykina, Yu. (2018). Potential of Eisenia andreiin complex cleaning of soils contaminated with fuel oil. Bulletin of Science and Practice, 4(10), 116—124. https://doi.org/10.5281/zenodo.1461875 [in Russian].

Tereshchenko, N. M., Kravets, O. V., Akimova, E. E. (2014). Victimization of peat and organic inputs to enhance systemic biological action to enhance the adaptogenic properties of plants. The achievements of science and technology in the agroindustrial complex, 28(12), 13—15. https://doi.org/10.32718/nvlvet-a9321 [in Ukrainian].

Chachina, S. B. (2014). Use of worms: rot worm (E. foetida) and Californian worm (E. andrei) for laying out industrial waste. Scientifi c newsletter of Omsk National University, 2(134), 217—223 [in Russian].

Mitina, N. B., Zubareva, I. M., Odina, A. D. (2016). Study on modifi ed sunfl ower husk fermentation process for ver micultivation. Food and environment safety, 2(15), 175—179. URL: http://fens.usv.ro/index.php/FENS/article/view/220 (Last accessed: 20.02.2024).

Aleksienko, O. M., Zhernosekova, I. V., Vinnikov, O. I. (2011). Infusion of exometabolites with Streptomyces recifensis var. liticus on the growth of the fungus Pleurotus ostreatus. Microbiology and biotechnology, 2(14), 41—47. https://doi.org/10.18524/2307-4663.2011.2(14).92772 [in Ukrainian].

Borzov, S. A., Nasonova, S. S., Polishchuk, O. V. (2017). Mathematical methods for modeling technical and biological systems with care. Dnipro [in Ukrainian].

Zhernosekova, I. V., Tymchuk, A. A., Ponizovtseva, A. G., Chernogor, N. P., Vinnikov, A. I. (2006). Biosynthetic characteristics of the mutant strain Streptomyces recifensis var. lyticus 2P-15 in the presence of exogenous aminoacids. Biosystems Diversity, 15, 33—38. https://doi.org/10.15421/010707 [in Ukrainian].

Dregval, O. A., Eremenko, A. O., Cherevach, N. V., Vinnikov, A. I. (2017). Antagonistic activity of soil streptomycetes in relation to plant pathogenic bacteria and fungi. Microbiology and biotechnology, 1, 73—84. https://doi.org/10.18524/2307-4663.2017.1(37).96323 [in Ukrainian].

Ivchenko, Y., Mitina, N. (2023). Infl uence of some physico-chemical factors on the biosynthesis of amylolitic enzymes of streptomycete origin. Biotechnologia Acta, 16(4), 59—65. https://doi.org/10.15407/biotech16.04.059 [in English].

Begunova, E. A. Stepnaya, O. A., Lysanskaya, V. Ya., Kulaev, I. S. (2003). The specifi city of the action of enzymes of the lysoamidase preparation on the cell walls of Staphylococcus aureus 209P. Biochemistry, 7, 896—901. https://doi.org/10.1023/A:1025074714910

Zhernosekova, I. V. (2012). Peculiarities of biosynthetic processes with streptomycetes due to the presence of plant species. Bulletin of Dnipropetrovsk University. Biology. Ecology, 2, 25—29. https://doi.org/10.15421/011224 [in Ukrainian].