The Influence of Microbial-Origin Growth Stimulators on the Development of Vermiculture
DOI:
https://doi.org/10.15407/scine20.04.061Keywords:
Keywords: vermiculture, Eisenia foetida culture, growth stimulants, culture fluid, substrate, biomass.Abstract
Introduction. The need to implement advanced technologies for obtaining a suffi cient amount of high-quality protein in Ukraine is an aspect of food security. Biotechnology of vermiculture is one of the ways to transform agricultural raw materials of plant and animal origin into good-quality food products.
Problems Statement. The search for ways to increase the productivity of protein biosynthesis in the process of vermiculture is an urgent problem.
Purpose. The purpose is to study the effect of microbial-origin stimulators in the nutritional substrate on the reproductive properties and biomass accumulation of Eisenia foetida.
Materials and Methods. Conventional and advanced physicochemical, microbiological, and technological research methods. Stimulators of microbial origin have been obtained by deep cultivation of Streptomyces recifensis var. lyticus 2435. The effect of stimulators has been studied by determining the biometric indicators of Eisenia foetida culture; the composition of the microbiota of the substrates has been investigated by the method of microscopy.
Results. The proposed growth stimulators are a complex of lytic enzymes (muramidase, 5 types of endopeptidase, 2 types of glycosidase) and accompanying enzymes (proteases, amylases). The maximum indicators have been found in the presence of 0.5% raw biomass of Streptomyces recifensis var. lyticus 2435 in a proportion of 1 : 15 to the dry matter of the nutrient substrate, the microbial composition of which changes against the initial values: the total number decreases by 13.38%, for the bacteria, by 13.92%, for the micromycetes, and by 31.04%, for the anaerobes. The number of representatives of the genus Streptomyces increases 2.17 times; there are no bacteria of the genii Enterobacter and Staphylococcus in the experimental samples. This fact is probably explained by the enzymatic activity of the digestive system of Е. foetida.
Conclusions. The studied stimulators of microbial origin have a positive effect on the nutrient substrate fermentation processes and on the reproductive and growth functions of vermiculture.
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