The Effect of the Gpc-B1 Gene on the Protein Content of Soft Winter Wheat Grain Against the Background of Genetic Environment of Ukrainian Varieties

Authors

DOI:

https://doi.org/10.15407/scine19.06.031

Keywords:

Key words: Triticum aestivum L.,, Gpc-B1 gene,, Triticum turgidum ssp. dicoccoides,, molecular markers,, grain protein content,, sedimentation rate.

Abstract

Introduction. The issue of improving grain quality is an urgent problem of wheat breeding. The transfer of genes from wild relatives is one of the directions of genetic improvement of wheat. The Gpc-B1 gene is of particular in terest in this direction.
Problem Statement. Using modern DNA marker systems to determine the Gpc-B1 gene from T. turgidum ssp. dicoccoides makes it possible to create new promising innovation varieties with increased protein content in combination with high economic and valuable properties in a short period of time.
Purpose. The purpose of our research is to determine the effect of the Gpc-B1 gene in the genetic environment of the Ukrainian variety Kuyalnyk on the grain protein content, yield, and bread-making characteristics.
Material and Methods. The Gpc-B1 gene has been detected by the method of multiplex polymerase chain reaction (PCR). The source of the Gpc-B1 gene, the Glu-Pro line, has been crossed with the Kuyalnyk variety (Gpc-B1 × Kuyalnyk). The protein content in grain has been measured by infrared spectrometry (NIR); the sedimentation index has been determined by the SDS-30 method, on an automatic device.
Results. Dominant and codominant molecular genetic systems of DNA markers have been developed to detect the Gpc-B1 gene from Triticum turgidum ssp. dicoccoides in soft winter wheat lines. The analysis of the grain of plants of generations F5—F9 has shown that the Gpc-B1 gene causes a 3% increase in protein content as compared with the original Kuyalnyk variety. The productivity of lines carrying the Gpc-B1 gene has been analyzed in field conditions.

Conclusions. The results of our research have shown that the influence of the Gpc-B1 gene on the grain yield is practically absent against the background of the genetic environment of the Kuyalnyk zoned Ukrainian variety. The influence of this gene on the baking properties of soft wheat has been studied. The lines with the Gpc-B1 gene and without it have almost the same sedimentation rate. We have created a modern breeding material of soft winter wheat with increased protein content in the grain in combination with high economic and valuable properties, which is ready for the state variety tests.

Downloads

Download data is not yet available.

References

Rybalka, O. I., Morhun, B. V., Polishchuk, S. S. (2018). Gpc-B1 (NAM-B1) gene as a new genetic resource in wheat breeding for increasing grain protein and micronutrient content. Fiziolohiya roslyn i henetyka, 50 (4), 279—298 [in Ukrainian]. https://doi.org/10.15407/frg2018.04.279

Alexandratos, N., Bruinsma, J. (2012). World agriculture: towards 2030/2050. The 2012 revision. Rome: ESA working paper.

Henchion, M., Hayes, M., Mullen, A., Fenelon, M., Tiwari, B. (2017). Future protein supply and demand: strategies and factors influencing a sustainable equilibrium. A review. Foods, 6 (7), 53. https://doi.org/10.3390/foods6070053.

Zhao, F., McGrath, S. (2009). Biofortification and phytoremediation. Curr. Opin. Plant Biol., 12, 373—380. https://doi.org/10.1016/j.pbi.2009.04.005

Pokhylko, S. Yu., Schwartau, V. V., Pochynok, V. M., Mykhalska, L. M., Duhan, O. M., Morgun, B. V. (2017). Comprehensive analysis of total protein content in soft wheat grain containing the Gpc-B1 gene from Triticum turgidum ssp. dicoccoides. Visnyk Ukrainskoho tovarystva henetykiv i selektsioneriv, 1, 52—57 [in Ukrainian]. https://doi.org/10.7124/visnyk. utgis.15.1.712.

Lundström, M., Leino, M.W., Hagenblau, J. (2017). Evolutionary history of the NAM-B1 gene in wild and domesticated tet raploid wheat. BMC Genet, 18, 118. https://doi.org/10.1186/s12863-017-0566-7

Nigarin, S., Shahidul, I., Juhasz, A., Ma, W. (2021). Wheat leaf senescence and its regulatory gene network. The Crop Jour nal, 9 (4), 1—15. https://doi.org/10.1016/j.cj.2021.01.004.

Sengar, R. S., Singh, A. (2018). Eco-friendly agro-biological techniques for enhancing crop productivity. Singapore: Springer Nature. https://doi.org/10.1007/978-981-10-6934-5

Wani, S. H., Mohan, A., Singh, G. P. (2021). Physiological, molecular, and genetic perspectives of wheat improvement. Cham: Springer Nature Switzerland AG. https://doi.org/10.1007/978-3-030-59577-7

Chen, X., Song, G., Zhang, S., Li, Y., Jie, G., Shahidul, I. … Ji, W. (2017). The allelic distribution and variation analysis of the NAM-B1 gene in Chinese wheat cultivars. J. Integr. Agric, 16, 1294—1303. https://doi.org/10.1016/S2095-3119(16)61459-4

Morgun, V. V., Rybalka, O. I., Morgun, B. V. (2021). New scientific directions of genetic improvement of cereal crops. Fiziolohiya roslyn i henetyka, 53 (3), 187—215 [in Ukrainian]. https://doi.org/10.15407/frg2021.03.187

Tabbita, F., Pearce, S., Barneix, A. (2017). Breeding for increased grain protein and micronutrient content in wheat: Ten years of the Gpc-B1 gene. J. Cereal Sci, 73, 183—191. https://doi.org/10.1016/j.jcs.2017.01.003

Distelfeld, A., Uauy, C., Fahima, T., Dubcovsky, J. (2006). Physical map of the wheat high-grain protein content gene Gpc-B1 and development of a high-throughput molecular marker. New Phytol, 169, 753—763. https://doi.org/10.1111/j.1469-8137.2005.01627.x

Uauy, C., Distelfeld, A., Fahima, T., Blechl, A., Dubcovsky, J. (2006). A NAC gene regulating senescence improves grain protein, zinc and iron content in wheat. Science, 314, 1298—1301. https://doi.org/10.1126/science.1133649

Venegas, J. P., Graybosh, R. A., Wienhold, B., Rose, D. J., Waters, B. M., Baenziger, P. S. … Amand, P. St. (2018). Biofortification of hard red winter wheat by genes conditioning low phytate and high grain protein concentration. Crop Science, 58, 1942—1953. https://doi.org/ 10.2135/cropsci2018.03.0175

Rybalka, O. I., Morgun, V. V., Morgun, B. V., Polishchuk, S. S. (2019). Genetic bases of the new direction of selection of wheat (Triticum aestivum L.) and triticale (Triticosecale Wittmack) classes that are original in terms of grain quality. Fizio lohiya roslyn i henetyka, 51 (3), 207—240 [in Ukrainian]. https://doi.org/10.15407/frg2019.03.207.

Pokhylko, S. Yu. (2018). Technological aspects of biofortification of soft wheat with the Gpc-B1 gene from Triticum turgidum ssp. dicoccoides. (PhD) (Techn.). Kyiv [in Ukrainian].

Singh, C., Srivastava, P., Sharma, A., Kaur, H., Sohu, V. S., Bains, N. S. (2019). Gpc-B1 mediated grain protein enhancement in wheat is compatible with high grain protein weight at moderated yield thresholds. Indian J. Genet., 79 (2), 494—498. https://doi.org/10.31742/IJGPB.79.2.14.

Classifier of quality indicators of botanical taxa, the varieties of which undergo examination for suitability for distribution. (2019). Vinnytsia: Ltd “Tvory” [in Ukrainian].

Patent of Ukraine № 65644. Rybalka, O. I., Pokoievoi H. V. Device for determination of sedimentation sds-30 [in Ukrainian].

Khan, I. A., Procunier, J. D., Humphreys, G. D. (2000). Development of PCR-based markers for a high grain protein content gene from Triticum turgidum ssp. dicoccoides transferred to bread wheat. Crop Science, 40, 518—524. https://doi.org/10.2135/cropsci2000.402518x

Distelfeld, A., Uauy, C., Fahima, T., Dubcovsky, J. (2006). Physical map of the wheat high-grain protein content gene Gpc-B1 and development of a high-throughput molecular marker. New Phytologist, 169, 753—763. https://doi.org/10.1111/j.1469-8137.2005.01627.x

Kim, Y., Kim, D., Shim, D. (2008). Expression of the novel wheat gene TM20 confers enhanced cadmium tolerance to ba kers’ yeast. Journal Biological Chemistry, 283, 15893—15902. https://doi.org/10.1074/jbc.M708947200

Downloads

Published

2023-12-22

How to Cite

MORGUN, B., SANDETSKA, N., & VELYKOZHON, L. (2023). The Effect of the Gpc-B1 Gene on the Protein Content of Soft Winter Wheat Grain Against the Background of Genetic Environment of Ukrainian Varieties. Science and Innovation, 19(6), 31–39. https://doi.org/10.15407/scine19.06.031

Issue

Section

Scientific and Technical Innovation Projects of the National Academy of Sciences