Development of Effective Strains of Nodule Bacteria and Microbial Preparations Based on Them




nodule bacteria, inoculation, bean-rhizobial symbiosis, strain, bacterial preparations, Rizostym


Introduction. Preparations based on highly effective and competitive strains of nodule bacteria improve nitrogen
and phosphorus nutrition of plants, are a source of biologically active compounds. They are environment friendly, have a high selective effect and aftereffect, increase the yield and enhance the stress-resistance of legumes.
Problem Statement. With the intensification of chemicalization of agriculture, the level of environment pollution is increasing while the quality of food is deteriorating. In this regard, it is important to search new, scientifically sound approaches to developing modern management systems that will ensure the production of environment
friendly plant products. The most advantageous way to solve this problem today is the optimization of plant-microbial interactions, one of the types of which is legume-rhizobial symbiosis.
Purpose. The purpose of this research is obtaining highly effective strains of rhizobia with the use of the cutting-edge molecular biology and nanobiotechnologies and developing innovative microbial preparations on their basis for inoculation of legume seeds.
Materials and Methods. Strains of nodule bacteria of alfalfa, goatweed, soybean, pea and lupine and S17-1
Escherichia coli strain with different plasmid vectors have been used. Methods of analytical selection, microbiological, physiological, and statistical have been applied.
Results. Highly effective competitive strains of rhizobia for basic legumes have been obtained. They provide
a yield increase of 11–21% as compared with the control strains. New generation preparations Rhizostym and Rhizostym-M, which are complex inoculants of binary action on the basis of nodule bacteria and additional biological agents, have been developed.
Conclusions. The developed microbiological innovative preparations provide a significant economic effect. They are used for obtaining environmentally friendly products, preserving and reclaiming soil fertility. These properties determine the prospects for their use in agricultural production.


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How to Cite

Kots, S. Y., & Morgun, V. V. (2021). Development of Effective Strains of Nodule Bacteria and Microbial Preparations Based on Them. Science and Innovation, 17(2), 39–49.



Scientific and Technical Innovation Projects of the National Academy of Sciences