DEVELOPMENT OF PROTOTYPES OF THERAPEUTIC AND PROPHYLACTIC ANTIOXIDANT AGENTS BASED ON NATURAL BIOLOGICALLY ACTIVE COMPOUNDS OF TROPICOGENIC REPRESENTATIVES OF ORCHIDACEAE JUSS.
Keywords:Orchidaceae Juss. extracts, polyphenolic compounds, nanosized silica, gelatin, and bioactive composites
Introduction. Plant extracts and bioactive substances (BAS) of natural origin possess a high biological activity and a low toxicity, therefore they are widely used in cosmetic, pharmaceutical, and food industries.
Problem Statement. Nowadays, about 50% of all medical drugs, dietary supplements, and herbal medicines is produced from natural sources, so the search for plants with a high content of BAS, the development of biotechnologies for BAS production, and the design of composites providing the most effective use of BAS are of great importance.
Purpose. The purpose is to study tropical orchid collection for identification of plants with a high BAS content; to develop a procedure for growing promising species in vitro; to design drug prototypes on the basis of plants extracts, nanosized silica, and gelatin biopolymer.
Materials and Methods. The extracts prepared from orchids leaves, silica, and gelatin have been used as auxiliary components for composites synthesis. Spectrophotometric, chromatographic, quantum-chemical, anatomo-morphological, physiological-biochemical, and biotechnological methods have been used in the research.
Results. The species of orchids, which contain a significant amount of BAS with high antioxidant properties have been identified, the main components of orchids extracts have been distinguished. Sterile cultures of selected species have been obtained; protocols for their cultivation in vitro have been developed. Two types of bioactive composites have been prepared; the interaction of extracts components with silica surface and gelatin molecules has been studied. It has been shown that the materials composed of silica and orchid extracts possess a high storage stability and a prolonged desorption of several BAS, while the materials based on extracts, silica, and gelatin ensure gradual release of all the extracts components.
Conclusions. The orchids are valuable sources of natural antioxidants. The designed composites are promising in terms of producing drug formulations for prolonged release of antioxidants.
Bandaranayake, W. M. (2006). Quality control, screening, toxicity, and regulation of herbal drugs. in Modern Phytomedicine. Turning Medicinal Plants into Drugs Weinheim (Eds. Ahmad I., Aqil F., Owais M.), Wiley-VCH GmbH & Co. KGaA, 25-57. https://doi.org/10.1002/9783527609987.ch2
Ekor, M. (2014). The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. J. Front Pharmacol., 4, 177. doi: 10.3389/fphar.2013.00177 https://doi.org/10.3389/fphar.2013.00177
Plant Extracts Market worth $59.4 billion by 2025. URL: https://www.marketsandmarkets.com/PressReleases/plantextracts.asp (Last accessed: 15.09.2019).
Katalinic, V., Milos, M., Kulisic, T., Jukic, M. (2006). Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chemistry, 94, 550-557. https://doi.org/10.1016/j.foodchem.2004.12.004
Proestos, C., Chorianopoulos, N., Nychas, G. J. E., Komaitis, M. (2005). RP-HPLC analysis of the phenolic compounds of plant extracts. Investigation of their antioxidant capacity and antimicrobial activity. J. Agric. Food. Chem., 53 (4), 1190-1195. https://doi.org/10.1021/jf040083t
Sasidharan, S., Chen, Y., Saravanan, D., Sundram, K. M., Yoga Latha, L. (2011). Extraction, isolation and characterization of bioactive compounds from plants' extracts. Afr. J. Tradit. Complement. Altern. Med., 8 (1), 1-10. https://doi.org/10.4314/ajtcam.v8i1.60483
Khan, M., Shaik, M. R., Adil, S. F., Khan, S. T., Al-Warthan, A., Siddiqui, M. R. H., Tahir, M. N., Tremel, W. (2018). Plant extracts as green reductants for the synthesis of silver nanoparticles: lessons from chemical synthesis. Dalton. Trans., 47, 11988-12010. https://doi.org/10.1039/C8DT01152D
Piminov, O. F., Shulga, L. I., Chihladze, K. A., Plys, S. V. (2015). Plant extracts in pharmacotherapy of stomatological diseases. Coliection of scientific works of staff member of P.L. Shupyk NMAPE, 24 (5), 176-185 [in Ukrainian].
Brand-Williams, W., Cuvelier, M. E., Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT, 28 (1), 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
Patent of Ukraine N 120415. Method for obtaining the hybrid silica-gelatin materials of increased hydrophobicity
How to Cite
Authors published in the journal “Science and Innovation” agree to the following conditions:
Authors retain copyright and grant the journal the right of first publication.
Authors may enter into separate, additional contractual agreements for non-exclusive distribution of the version of their work (article) published in the journal “Science and Innovation” (for example, place it in an institutional repository or publish in their book), while confirming its initial publication in the journal “Science and innovation.” Authors are allowed to place their work on the Internet (for example, in institutional repositories or on their website).