DEVELOPMENT OF PROTOTYPES OF THERAPEUTIC AND PROPHYLACTIC ANTIOXIDANT AGENTS BASED ON NATURAL BIOLOGICALLY ACTIVE COMPOUNDS OF TROPICOGENIC REPRESENTATIVES OF ORCHIDACEAE JUSS.

Authors

DOI:

https://doi.org/10.15407/scine17.01.089

Keywords:

Orchidaceae Juss. extracts, polyphenolic compounds, nanosized silica, gelatin, and bioactive composites

Abstract

Introduction. Plant extracts and bioactive substances (BAS) of natural origin possess a high biological activity and a low toxicity, therefore they are widely used in cosmetic, pharmaceutical, and food industries.

Problem Statement. Nowadays, about 50% of all medical drugs, dietary supplements, and herbal medicines is produced from natural sources, so the search for plants with a high content of BAS, the development of biotechnologies for BAS production, and the design of composites providing the most effective use of BAS are of great importance.

Purpose. The purpose is to study tropical orchid collection for identification of plants with a high BAS content; to develop a procedure for growing promising species in vitro; to design drug prototypes on the basis of plants extracts, nanosized silica, and gelatin biopolymer.

Materials and Methods. The extracts prepared from orchids leaves, silica, and gelatin have been used as auxiliary components for composites synthesis. Spectrophotometric, chromatographic, quantum-chemical, anatomo-morphological, physiological-biochemical, and biotechnological methods have been used in the research.

Results. The species of orchids, which contain a significant amount of BAS with high antioxidant properties have been identified, the main components of orchids extracts have been distinguished. Sterile cultures of selected species have been obtained; protocols for their cultivation in vitro have been developed. Two types of bioactive composites have been prepared; the interaction of extracts components with silica surface and gelatin molecules has been studied. It has been shown that the materials composed of silica and orchid extracts possess a high storage stability and a prolonged desorption of several BAS, while the materials based on extracts, silica, and gelatin ensure gradual release of all the extracts components.

Conclusions. The orchids are valuable sources of natural antioxidants. The designed composites are promising in terms of producing drug formulations for prolonged release of antioxidants.

Downloads

Download data is not yet available.

References

Bandaranayake, W. M. (2006). Quality control, screening, toxicity, and regulation of herbal drugs. in Modern Phytomedicine. Turning Medicinal Plants into Drugs Weinheim (Eds. Ahmad I., Aqil F., Owais M.), Wiley-VCH GmbH & Co. KGaA, 25-57. https://doi.org/10.1002/9783527609987.ch2

Ekor, M. (2014). The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. J. Front Pharmacol., 4, 177. doi: 10.3389/fphar.2013.00177 https://doi.org/10.3389/fphar.2013.00177

Plant Extracts Market worth $59.4 billion by 2025. URL: https://www.marketsandmarkets.com/PressReleases/plantextracts.asp (Last accessed: 15.09.2019).

Katalinic, V., Milos, M., Kulisic, T., Jukic, M. (2006). Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chemistry, 94, 550-557. https://doi.org/10.1016/j.foodchem.2004.12.004

Proestos, C., Chorianopoulos, N., Nychas, G. J. E., Komaitis, M. (2005). RP-HPLC analysis of the phenolic compounds of plant extracts. Investigation of their antioxidant capacity and antimicrobial activity. J. Agric. Food. Chem., 53 (4), 1190-1195. https://doi.org/10.1021/jf040083t

Sasidharan, S., Chen, Y., Saravanan, D., Sundram, K. M., Yoga Latha, L. (2011). Extraction, isolation and characterization of bioactive compounds from plants' extracts. Afr. J. Tradit. Complement. Altern. Med., 8 (1), 1-10. https://doi.org/10.4314/ajtcam.v8i1.60483

Khan, M., Shaik, M. R., Adil, S. F., Khan, S. T., Al-Warthan, A., Siddiqui, M. R. H., Tahir, M. N., Tremel, W. (2018). Plant extracts as green reductants for the synthesis of silver nanoparticles: lessons from chemical synthesis. Dalton. Trans., 47, 11988-12010. https://doi.org/10.1039/C8DT01152D

Piminov, O. F., Shulga, L. I., Chihladze, K. A., Plys, S. V. (2015). Plant extracts in pharmacotherapy of stomatological diseases. Coliection of scientific works of staff member of P.L. Shupyk NMAPE, 24 (5), 176-185 [in Ukrainian].

Brand-Williams, W., Cuvelier, M. E., Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT, 28 (1), 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5

Patent of Ukraine N 120415. Method for obtaining the hybrid silica-gelatin materials of increased hydrophobicity

Additional Files

Published

2021-03-03

How to Cite

Ivannikov Р., Laguta І., Stavinskaya О., & Buyun Л. (2021). DEVELOPMENT OF PROTOTYPES OF THERAPEUTIC AND PROPHYLACTIC ANTIOXIDANT AGENTS BASED ON NATURAL BIOLOGICALLY ACTIVE COMPOUNDS OF TROPICOGENIC REPRESENTATIVES OF ORCHIDACEAE JUSS. Science and Innovation, 17(1), 89–95. https://doi.org/10.15407/scine17.01.089

Issue

Section

Scientific and Technical Innovation Projects of the National Academy of Sciences