Influence of the Conditions of Alkaline Pretreatment of Vegetable Raw Material before Hydrolysis in a Rotor Pulsation Device on the Cellulose Conversion

Ya. BLUME; O. OBODOVYCH; V. SYDORENKO

doi:10.15407/scine20.06.030

Authors

Ya. BLUME Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine https://orcid.org/0000-0001-7078-7548
O. OBODOVYCH Institute of Engineering Thermophysics of the National Academy of Sciences of Ukraine https://orcid.org/0000-0001-7213-3118
V. SYDORENKO Institute of Engineering Thermophysics of the National Academy of Sciences of Ukraine https://orcid.org/0000-0001-7735-7719

DOI:

https://doi.org/10.15407/scine20.06.030

Keywords:

fuel ethanol, pretreatment, alkali, cellulose conversion, cellulase complex

Abstract

Introduction. The growing global energy demand, limited fossil fuel resources, and increasing greenhouse gas
emissions highlight the need for expanded use of renewable energy sources, particularly biomass.
Problem Statement. A significant drawback in the production of bioethanol is its high cost, primarily due to
the presence of hemicellulose and lignin.
Purpose. This study aims to determine the effect of treating an alkaline suspension of wheat straw in a rotary pulsation device on the degree of cellulose conversion during enzymatic hydrolysis, a critical step in the production
of fuel ethanol.
Materials and Methods. The raw material is pre-crushed wheat straw. The experiments have been conducted
with the use of a rotary pulsation device in an experimental plant.
Results. It has been found that the combined physical effects of the discrete pulse energy input method and
reduced straw particle size significantly increase the lignin extraction, from 40.0% at an average particle size of
2—1 mm to 62.0% at an average particle size of 0.4—0.1 mm. Additionally, the treatment of an alkaline suspension of wheat straw at 90 ^оC for one hour, at alkali concentrations ranging from 1 to 4% (wt./wt.), results in an
increase in the cellulose conversion during enzymatic hydrolysis from 38% to 65.8%. Additionally, a corresponding decrease in the lignin content, from 17.1% to 3.16% has been reported.
Conclusions. Increasing alkali concentration during the alkaline pretreatment of wheat straw using the discrete-pulse energy input method in a rotary pulsation device enhances the rate of cellulose conversion during
subsequent enzymatic hydrolysis.

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