Development of Glutathione Production Technology Based on Designed Active Yeast Overproducers

1Yurkiv, MT
1Kurylenko, OO
1Vasylyshyn, RV
2Dmytruk, KV
3Martynyuk, NB
3Skorohod, VV
2Sybirny, AA
1Institute of Cell Biology, the NAS of Ukraine, Lviv
2Institute of Cell Biology, NAS of Ukraine, Lviv
3Enzyme Joint-Stock Company, Ladyzhyn
Sci. innov. 2015, 11(5):53-60
https://doi.org/10.15407/scine11.05.053
Section: Research and Engineering Innovative Projects of the National Academy of Sciences of Ukraine
Language: English
Abstract: 

The Hansenula polymorpha recombinant strain overexpressing both GSH2 gene encoding γ-glutamyl-cysteine-synthetase and MET4 gene encoding the transcription activator of genes involved in cysteine (precursor of glutathione) biosynthesis has been obtained using metabolic engineering approaches. The recombinant strain is characterized by significantly increased glutathione output as compared with in vitro wild-type strain. Conditions for efficient glutathione production by recombinant H. polymorpha strain have been optimized. A semi-industrial model for glutathione production using the designed H. polymorpha overproducer has been developed.

Keywords: and metabolic engineering., glutathione, Hansenula polymorpha, yeast
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